Study on species diversity of mushrooms in the botanical area Thung Khai province. The researching range from June to August and November 2558 BC The 2558 to January 2005. 2559 total time survey and sample mushroom, as 6 months in space exploration 2 route by route 1 cycling route. The distance 5 kilometers and the path 2 nature trail bridge canopy wood distances 3 kilometers. Survey and sampling method of recording. The record label mushroom found a friend to bring population and establishing a roster of mushrooms. The species diversity of mushrooms in the botanical area Thung Khai province. Can arrange classification has 6 Order 16 Family 27 species. Found in species diversity of mushrooms found 3 rank is a mushroom in the family, and Agaricales Polyporales Pezizales and can identify mushrooms mushrooms can be eaten. And mushrooms that cannot be eaten, 27 species Can be divided into the mushroom can eat can be classified 15 species. And mushrooms can eat can be classified 12 species.