Based on this finding, we used a Restek column—the 30m,
0.25mm ID, 0.25 μm FAMEWAX™ column (cat.# 12497)—to
analyze these oils. The FAMEWAX™ column contains a
polyethylene glycol stationary phase, which is slightly more
polar than the Stabilwax® column. The FAMEWAX™ column
offers excellent selectivity and efficiency, not only to separate
saturated (C16:0 and C18:0) and monounsaturated (C18:1n9)
fatty acids from the Omega-3 and -6 fatty acids of interest, but
also to resolve the isomers of linolenic acid (C18:3n3 and
C18:3n6).