The aim of this work was to study the influence of polyglycerols on the selected properties of water-based printing ink and prints overprinted with water-based flexographic printing inks. Modified flexographicinks were laboratory printed on polyethylene plastic film. The impact of small amounts of various com-mercially available polyglycerols on printing ink colour was examined for the optical density of thefull-tone area, colour values (CIELAB) and total colour difference E∗aband the gloss of the dried inkfilm. The dry and wet rub resistance of overprinted samples was investigated. In general, the additionof polyglycerols improved the wet and rub resistance of water-based flexographic printing ink with anacceptable total colour difference E∗ab. The optical density and the gloss increased for those printing inkscontaining polyglycerols as compared to original printing ink. The small addition of glycerol ethoxylateto the water-based printing ink improved the wettability of the plastic film and the wet and dry rubresistance.