The particle size distribution of unilanlellar vesicles prepared byextrusion is ?;-a function of the number of passes through the Extruder Membrane. Hy- 0(/)drated lipid solutions will initially form large, multi-lamellar vesicles. After 55the initial pass through a membrane, the particle size distribution will tend Etowards a binlodal distribution. After sufficient passes through the mem- -brane, a unimodal, normal distr!bution is obtained. A minimum of elevenpasses through the Extruder membrane is recommended for most lipids.N4 The Plus particle submicron size information particle size in ~ Figure analyLer. _2 was The obtaine<;i particle size with analyzer a Coulter wascontrolled through a PC usingthe N4 Plus software package. The Coulter-..~, may be contacted at (800) 338-8830.Glycero-3-Phosphocholinewas made upparticle size distribution was measured.for 3 and 11 passes through a..A solution of l,2-Dioleoyl-snto25 mg/ml in Dl water. -for the material before extrusionlOOnm (0. 1 micron) membrane.
การแปล กรุณารอสักครู่..
