All eggs were weighed, in pools of ten eggs, before; heating, incubation, and transfer to the hatcher to evaluate the egg weight loss. Egg weight loss was determined during the storage periods, after 18 days of incubation and the total of two periods. After 18 days of incubation, all eggs were candled to calculate the fertility percentage, for each replicate (10 eggs each). Eggs with evidence of living embryos were transferred from the incubation trays to the hatcher trays. After the hatch was completed, un-hatched eggs were removed, broken and submitted to embryo diagnosis to determine the percentages of early, intermediate, and late embryo mortality (1 to 7, 8 to 14 and 15 to 21 days of embryo development, resp.). Early dead embryos were differentiated by the absence of an egg tooth; the intermediate dead were differentiated by the presence of an egg tooth,