Fig. 2. Flavobacterium columnare, Edwardsiella ictaluri and
Aeromonas hydrophila multiplex-PCR amplified products resolved
by agarose gel electrophoresis showing the lowest
level of detection of target nucleic acid from F. columnare,
E. ictaluri and A. hydrophila. Lane 1: 123 bp DNA ladder
(Invitrogen) as size standard. Amplicons generated = Lane 2:
20 ng of each template; Lane 3: 2 ng of each template; Lane 4:
200 pg of each template; Lane 5: 20 pg of each template;
Lane 6: 2 pg of each template; Lane 7: 200 fg of each template;
Lane 8: 20 fg of each template; Lane 9: 2 fg of each template.
504 bp amplicon amplified from F. columnare DNA, 407 bp
amplicon from E. ictaluri DNA, and 209 bp amplicon from
A. hydrophila DNA