2.6. SPAD index and electrolyte leakage determination
On May 13 (64 days after transplanting), a chlorophyll meter (SPAD-502, Minolta corporation, Ltd., Osaka, Japan) was used to take readings from the fully expanded leaves. Fifteen leaves per experimental unit were randomly measured and averaged to a sin- gle SPAD value for each treatment.
Electrolyte leakage (EL) was determined as described by Lutts et al. (1995). Briefly, six randomly chosen plants per treatment were taken and cut into 1-cm segments. Leaf samples were rinsed with distilled water, and then placed in stoppered glass vials con- taining 10 mL of deionized water. The samples were incubated at
room temperature (25 ◦C) on a shaker for one day. Electrical con-
ductivity of the bathing solution (EC1 ) was measured directly after incubation. The same samples were then placed in an autoclave at
120 ◦C for 20 min and a second measurement of the EC (EC2 ) was
made after cooling the solution. The EL expressed as percentage was calculated as the ratio of EC1 /EC2 .
2.6. SPAD index and electrolyte leakage determinationOn May 13 (64 days after transplanting), a chlorophyll meter (SPAD-502, Minolta corporation, Ltd., Osaka, Japan) was used to take readings from the fully expanded leaves. Fifteen leaves per experimental unit were randomly measured and averaged to a sin- gle SPAD value for each treatment.Electrolyte leakage (EL) was determined as described by Lutts et al. (1995). Briefly, six randomly chosen plants per treatment were taken and cut into 1-cm segments. Leaf samples were rinsed with distilled water, and then placed in stoppered glass vials con- taining 10 mL of deionized water. The samples were incubated atroom temperature (25 ◦C) on a shaker for one day. Electrical con-ductivity of the bathing solution (EC1 ) was measured directly after incubation. The same samples were then placed in an autoclave at120 ◦C for 20 min and a second measurement of the EC (EC2 ) wasmade after cooling the solution. The EL expressed as percentage was calculated as the ratio of EC1 /EC2 .
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