and nuclease free water. The amplification was done with an initial denaturation step at 95 °C for 3 min, followed by 30 cycles of amplification at 95 °C for 1 min denaturation, 55 °C annealing for 1 min, and 72 °C extension for 1 min with a final extension step at 72 °C for 7 min. The PCR products (∼360 bp) were separated using electrophorator on 1% (w/v) agarose gel and visualized using G:BOX gel documentation