Therefore, in this project, we aimed to investigate the effect of electrospinningparameters, including CA concentration, voltage, and spinning distance, on the electrospinnability of CA nanofibers and fiber diameter, using process optimization principles and response surface methodology. Certainprocess parameters, including solvent (acetone), needle diameter (gauge 22, inner diameter 0.413 mm), temperature (20 C), and feed rate (2 mL/h), were kept constant throughout the experiment. Preliminary experimentswere conducted to determine the extreme conditions of each parameter and to define a working boundary. Then,trials of electrospinning CA nanofibers were conducted following a 3-factor, 3-level Box-Behnken design withinthe predetermined range. CA nanofibers morphology was characterized by scanning electron microscope (SEM)and ImageJ software to obtain their mean diameter. The mean electrospun fiber diameters fell into the range from404 to 1346 nm and increased with increasing CA concentration. The fiber diameter was impacted less significantly by the other two parameters, i.e., voltage and spinning distance.