The thermostability characteristics of cellulases enzyme system
is a key to industrial interest of cellulose hydrolysis. Thermostable
cellulolytic enzymes have wide applications in food and sugar
industries where high-temperature process such as pasteurization
is used [1]. The enzymatic hydrolysis of lignocellulosic waste
and fermentation of reducing sugars for production of biofuel and
desired metabolites is a promising strategy for efficient utilization
of renewable resources [2]. Pure form of substrate such as
Aviel, Solka Flock, and cotton are expensive feed stock used by
the researchers for large scale cellulase production [3,4]. A significant
reduction in cost will be important for their commercial
use by cellulase based strategies: to increase commercial enzymes
volumetric productivity, producing enzymes using cheaper substrates
[5]. The enzymatic hydrolysis of cellulose is a complex
reaction with many factors and changing dynamics in concert apart
in the overall reaction [6]. Several researches have focused on
enhancing cellulase activity to improve the yield and rate of enzymatic
hydrolysis [7–9]. The hydrolysis of lignocellulosic biomass
is not substrate specific; the crude enzyme mixture can be more
efficient than the isolated enzymes [10]. Utility cost of enzymatic
hydrolysis is low as compared to acid or alkaline hydrolysis