Avocado mesocarp (1 g) was
homogenised with 4 ml of distilled water and then centrifuged at
10,000g for 20 min at 4 C, and 1.58 ml of distilled water was
added to 0.02 ml of the supernatant; then 0.1 ml of Folin–Ciocalteau’s
reagent was added. The reaction was neutralised by adding
0.3 ml of 20% (w/v) sodium carbonate.