This work focuses on the production of antihypertensive and antioxidant activities using enzymatic
hydrolysis of protein concentrates recovered by ultrafiltration of different wastewaters from the industrial
processing of cuttlefish (Illex argentinus). The effluents were produced in the processes of thawing
(E1), softening (E2), boiling (E3) and gelation (E4). Our results showed that membranes with cut-off at
100, 30 and 10 kDa were an effective resource to protein concentration of E2 and E3 but limited for E1 and
E4. In addition, E2 and E3 retentates led to remarkable antihypertensive and antioxidant activities, further
improved by enzymatic hydrolysis. Also sequential ultrafiltration revealed the enrichment of these
protein concentrates in peptides with high angiotensin-converting enzyme (ACE)-inhibitory activity.
Thereby, UF-fractionation followed by proteolysis of protein concentrates from cuttlefish wastewaters
offers new opportunities for the development of bioactive hydrolysates with application in the food
industry. In addition, this approach contributes to an improved depuration of industrial wastewaters,
reducing the treatment costs and leading to a decrease in its contaminating effect.
This work focuses on the production of antihypertensive and antioxidant activities using enzymatichydrolysis of protein concentrates recovered by ultrafiltration of different wastewaters from the industrialprocessing of cuttlefish (Illex argentinus). The effluents were produced in the processes of thawing(E1), softening (E2), boiling (E3) and gelation (E4). Our results showed that membranes with cut-off at100, 30 and 10 kDa were an effective resource to protein concentration of E2 and E3 but limited for E1 andE4. In addition, E2 and E3 retentates led to remarkable antihypertensive and antioxidant activities, furtherimproved by enzymatic hydrolysis. Also sequential ultrafiltration revealed the enrichment of theseprotein concentrates in peptides with high angiotensin-converting enzyme (ACE)-inhibitory activity.Thereby, UF-fractionation followed by proteolysis of protein concentrates from cuttlefish wastewatersoffers new opportunities for the development of bioactive hydrolysates with application in the foodindustry. In addition, this approach contributes to an improved depuration of industrial wastewaters,reducing the treatment costs and leading to a decrease in its contaminating effect.
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