The quantification of soluble VCAM-1 and soluble ICAM-1
was determined using commercially available ELISA kit from
USCN Life Science Inc. (Wuhan, China) and Abnova (Taipei,
Taiwan), respectively following manufacturer’s instruction.14
Absorbance at 450 nm was determined using VERSA micro
plate reader (USA). Values of samples were calculated by the
software provided with the micro plate reader and compared
to the standard curve generated.