Composition of the FAME was analyzed with gas chromatograph-mass spectrometry (GC-MS QP2010 Plus, Shimadzu Corporation, Japan) equipped with a flame ionization detector (FID) and a capillary column 30m × 0.32mm × 0.25µm (DB-WAX, Carbowax 20M). Yield of biodiesel was calculated by Equation (1).