HPLC system (Shimadzu LC-20AT, Tokyo, Japan) consisting of
Model LC-20AT multi-solvent delivery system and Model
RF-10AXL fluorescence detector were used for the analysis. For
recording and data processing, LC Solution software from
Shimadzu (Tokyo, Japan) was used.
The analytical column, Lichrospher 60 RP-Select B
(250mm 4 mm), 5 lm of pore size, was supplied from Agilent
(Santa Clara, CA, USA).