In terms of cost, the new PCR protocol established in this
study is more economical than the previous protocol since no
commercial kit is needed (15). Regarding the sample-processing
time required to complete the PCR test, the protocol took
approximately 8 h while the previous protocol using the commercial
stool kit took approximately 7 h. The new protocol
needed an hour longer for proteinase K digestion and phenolchloroform
extraction.