HPLC grade cyclohexane, dichloromethane, methanol and
deionized water were used to fractionate soluble compounds from
the mushrooms in ascending polarity by sequentially extracting
2e100 g (depending on material availability) ground mushrooms in
a Soxhlet extractor for 5 h. The samples were air dried after each
solvent extraction and finally the residues were extracted with
boiling water during 5 h constantly mixing in the Ikamag ‘‘RTC
basic” magnetic stirrer (IKA Labortechnik, Staufen, Germany).
Organic solvents were removed in a vacuum rotary evaporator RV
10 (IKA, Staufen, Germany), while water extracts were freeze-dried.
All extracts were kept in a refrigerator until further analysis