To detect the level
of virulence of the obtained Aeromonas hydrophila
strain, laboratory test were conducted using Nile tilapia
(O. niloticus) as the fish of choice. According to
Wakabayashi, et. al (1981) the Bacterial solution for
fish inoculation was prepared by suspending 20 hs
culture from brain heart infusion agar of the obtained
strain in sterile physiological saline solution to give a
concentration of 5 mg bacterial cells by wet weight/ml
which was estimated to be between 1.8 / 106 and 1.6 /
109 C.F.U/ml .