Previous studies using other cell lines reported effects of single FAs that were much greater than our findings. Hence, the types of human intestinal cell lines used in the different studies seem to be a likely reason for this. A study comparing the cell lines HCT 116 and HT29 exposed to 2mM butyrate showed that ANGPTL4 expression in HCT 116 cells and HT29 cells were increased by threefold and hundredfold, respectively (Korecka et al., 2013).