Abstract: Several trials for induction of Sisymbrium irio L. callus were carried out on Murashige and
Skoog (MS) medium supplemented with different concentrations of auxins and kinetin. Maintenance
of calli on MS medium treated with Spirulina aqueous extract (SAE) as a source of vitamins and
phytohormones was achieved. Growth and growth parameters of calli revealed that an obvious
extension in the exponential phase with variable enhancement of the growth rate by 1 to 4 times more
d than irrespective control. Doubling time (t ) and the relative growth rate (RGR) of callus were also
estimated at different treatments. Antioxidant capacities of calli grown on MS medium supplemented
with different SAE were enhanced up to 12 times more than control calli. The previous results were
coincidentally with an obvious accumulation of both flavonoids and total phenolic compounds in
cultures treated with SAE. The complementary between the previous results manifested the longevity
and viability of calli on modified MS medium. Antioxidant activity also confirmed the different
results. Successful suspension cultures were established using MS medium without vitamins and
phytohormones and supplied with 18% SAE. Growth parameters, total flavonoids, phenolic compounds
and antioxidant activity were highly increased compared with the untreated cultures.