After incubation, the bags were removed, washed with water until clean and dried in a ventilated oven at 55°C for 72 h, then removed and oven-dried again at 105°C. To estimate the faecal DM excretion, indigestible neutral detergent fibre (IaNDF) was used as an internal marker (Zeoula et al., 2002). The IaNDF was estimated using the difference between weight before and after ruminal incubation of feed and faeces according Zeoula et al. (2002).