The high-throughput screening was performed using HT Protein Express 200 Chip with a total volume of 40 μL. The protocol for the analysis of proteins was followed according to the manufacturer’s instructions (Caliper Life Sciences,
Hopkinton, MA, USA). The high detection sensitivity was possible by utilizing fluorescent dyes that bind SDS–protein
complexes. The assay itself relies on internal protein standards to determine the protein size and concentration. The
soybean protein variations were identified and selected according to their molecular weight, and the data were processed using Microsoft Excel.