Fig. 1.
In vitro regeneration of nodal explants derived from mature tree of H. opposita. (A) Nodal explants with multiple shoot development on MS medium supplemented with 4.4 μM BA after 30 days of culture. (B) Stunted and malformed shoots developed on MS medium supplemented with 4.7 μM Kin after 30 days of culture. (C) Development of roots from the base of an in vitro regenerated shoot on MS medium supplemented with 3.6 μM IAA after 21 days. (D) Three-month-old regenerated plants in the greenhouse