Subjects: Sixty male volunteers were assigned to three groups according to age. There were twenty male volunteers, in each of the three groups (Group-A: 60-69 years; Group-B: 50-59 years and Group-C: 40-49 years). Each group was again equally sub-divided at random into one experimental group (n=10) and one control group (n=10). Subjects were recruited from the vicinity of Kalyani University, Kalyani, West Bengal, India.