All the sequences obtained were aligned using MEGA 3.1
software. In order to test the performance of the mini-barcodes in
species identification, we calculated the variable and parsimony
informative sites and the nucleotide divergences (using Kimura-2
parameter) between species in both the barcode datasets obtained
from the two different primer sets, in MEGA 3.1 software. To
identify the species, the sequences were subjected to similarity
searches with the reference sequences in both the NCBI (http://
blast.ncbi.nlm.nih.gov/Blast.cgi) and the BOLD systems