Antagonism of endophytic bacteria
against Colletotrichum spp.
Lyophilized fungal cultures were
purchased from MTCC Chandigarh.
Cultures were grown in laboratory as per
the instructions and specific media given
by MTCC Chandigarh. Chilli red fruit
endophytic bacterial isolates were assayed
for antifungal activities against
C.gloesporioides (MTCC3439), by dual
culture method using Potato Dextrose
Agar (PDA) medium. Endophytic isolates
were streaked on PDA medium 3 cm in
distance opposite to pathogenic fungi
inoculated at the centre of the medium.
The barrier between endophytic isolate
and fungi indicated the antagonist
interaction between them. Antagonist
activity was investigated for 4 to 7 days
after incubation at room temperature. The
value of inhibition was measured using the
Int.J.Curr.Microbiol.App.Sci (2014) 3(2): 318-329
320
formula 1-(a/b) x 100% (a: distance
between fungi in the centre of Petri dish to
endophytic isolate, b: distance between
fungi in the center of Petri dish to blank
are without endophytic isolate) described
by Kumar et al. (2002)