2.5. Analysis methods
The concentration of pigments is usually represented by color
value based on the absorbance spectrum of Monascus pigments
at their characteristic wavelength [19]. Based on the spectrum of
Monascus pigments, the absorbance of Monascus pigments at the
specific wavelength was used to represent the pigment concentration,
such as absorbance at 470 nm corresponding to the characteristic
absorbance of orange Monascus pigments. The composition of
Monascus pigments was further validated by thin layer chromatography
(TLC) analysis using Silica gel 60 F254 TLC plate (Merck).
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2.5. Analysis methods
The concentration of pigments is usually represented by color
value based on the absorbance spectrum of Monascus pigments
at their characteristic wavelength [19]. Based on the spectrum of
Monascus pigments, the absorbance of Monascus pigments at the
specific wavelength was used to represent the pigment concentration,
such as absorbance at 470 nm corresponding to the characteristic
absorbance of orange Monascus pigments. The composition of
Monascus pigments was further validated by thin layer chromatography
(TLC) analysis using Silica gel 60 F254 TLC plate (Merck).