The LAB concentration (MRS) at 30 days was quite different
among the brine samples analysed, and as expected, the highest
counts were registered in the samples inoculated with LAB starters
(‘‘i’’ samples). Lactic acid bacteria were always present, their count
was quite constant up to 90 days, reaching a value higher than 6
Log CFU mL1 at the end of sampling, after six months of fermentation,
with the exception of Nocellara del Belice (4.5 Log
CFU mL1) and Carolea (2.0 Log CFU mL1) control samples.
Mesophilic aerobic bacteria counts (PCA) showed a similar
trend in all samples, slightly increasing up to the end of sampling
reaching a value higher than 7 Log CFU mL1 (the concentration
was 1–2 Log10 units higher than that registered at 30 days).
The initial level of yeasts and moulds (SAB) was lower than total
aerobic bacteria (PCA) counts. Yeasts and moulds dynamics
throughout fermentation were quite similar among samples,
showing an increasing trend after 30 days of fermentation.
Moreover, the three variables (time, cultivar and starter) and
their interactions were statistically significant (P < 0.01) when
the microbial results were subjected to the multivariate analysis.
The LAB concentration (MRS) at 30 days was quite differentamong the brine samples analysed, and as expected, the highestcounts were registered in the samples inoculated with LAB starters(‘‘i’’ samples). Lactic acid bacteria were always present, their countwas quite constant up to 90 days, reaching a value higher than 6Log CFU mL1 at the end of sampling, after six months of fermentation,with the exception of Nocellara del Belice (4.5 LogCFU mL1) and Carolea (2.0 Log CFU mL1) control samples.Mesophilic aerobic bacteria counts (PCA) showed a similartrend in all samples, slightly increasing up to the end of samplingreaching a value higher than 7 Log CFU mL1 (the concentrationwas 1–2 Log10 units higher than that registered at 30 days).The initial level of yeasts and moulds (SAB) was lower than totalaerobic bacteria (PCA) counts. Yeasts and moulds dynamicsthroughout fermentation were quite similar among samples,showing an increasing trend after 30 days of fermentation.Moreover, the three variables (time, cultivar and starter) andtheir interactions were statistically significant (P < 0.01) whenthe microbial results were subjected to the multivariate analysis.
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