Cadaminelyrata, aquatic plant, is use for decoration on a small aquarium. The present study was to increase the number of them by tissue culture. Shoot explant were excised and cultured on solid, liquid and semi-solid Murashige and Skoog (MS) medium supplemented with 0.5 mg/l NAA (α-naphthaleneacetic acid) and 2 mg/l BA (N6-benzyladenine) with or without 0.2% activated charcoal (AC). The cultures were placed under light conditions at 14 h photoperiod, 27±1 oC to initiate callus induction and plant regeneration for 3 months. The result revealed that semi-solid MS medium supplemented with0.5 mg/l NAA and 2 mg/l BA without 0.2% AC gave the callus induction (100%)and size of callus at 1.06 centimeter better than another type of culture media.Liquid MS medium supplemented with 0.5 mg/l NAA and 2 mg/l BA with or without 0.2% AC, gave the highest average number of shoot at 68.80/explant and shoot length at 10.69 centimeter,significant different with another type of culture media. This result showed that AC and type of culture media is improper for callus induction and shoot number.