defining different factors affecting the efficiency of in
vitro plant regeneration. In an experiment for I. hawkerii,
shoot formation rate was the highest in MS medium
(Murashige and Skoog, 1962) with 0.1 μM N-(2-chloro-
4-pyridil)-N’-phenylurea–forchlorfenuron (CPPU)
(Milošević et al., 2011). A similar result was found by
Subotić et al. (2008). They investigated the regeneration
of axillary buds of I. walleriana in culture in vitro. The
media with 0.1 μM CPPU induced the highest number
of axillary buds and roots per explant.