Still, other studies especially on gonadal development especially on the sex-changing period [29,32] is necessary for TGGG hybrid grouper.
With this, female, sex-changing period and male of TGGG hybrid grouper can be etermined. Results of this study suggested that the TGGG hybrid grouper could reach sexual maturation as evident from development of their gonads. This opens up the possibility of using the hybrid progeny to develop F2 generation and performing backcross breeding.