A method for the determination of water-soluble vitamins in several energy and sport drinks by micellar
electrokinetic chromatography (MEKC) has been developed in this work. The separation of vitamins was
studied in terms of background electrolyte composition (borate content, pH, surfactant type and content)
and in other MEKC parameters. A study of the possible compounds found in the vitamin-enriched drinks
that could interfere in vitamin determination was also performed, and a modified procedure with
enhanced resolution was developed. The proposed method was successfully applied to the analysis of
water-soluble vitamins in a variety of energy and sport drinks and also in fruit nectars. The method
implies minimal sample preparation and reagent consumption, being environmentally sustainable. Thus,
the proposed methodology could be useful for quality control purposes in the soft drink industry.