Aluminum hydroxide gel, Montanide and Quil A adjuvants are frequently used in commercial veterinary vaccine, and were selected for this study because they are well tolerated and induce fast and sustainable humoral and cell-mediated immune reponses, essential for protection against a rough Brucella. In addition, we tested a IFA bacause it is a classical adjuvant that induce potent immune responses. We administered these vaccine subcutaneously, since this route is commonly used in dogs. In addition , we tested previously used DNA and prime-boost strategies.