2.5. Particle Size Distribution and Polydispersity Index To calculate the average particle size and polydispersity, the dynamic light scattering technique was used (Mal- vern 4700 MW, Spectra-Physics 127 model) at a wave- length of 632.8 nm, coupled to the BI-200M version 2.0 goniometer and BI-9000AT digital correlator from Brookheaven Instruments [25]. Polydispersity evaluates the size distribution of particles, showing the suspen- sion’s degree of homogeneity. The liposomes were fil- tered through 0.45 μm filter paper and two drops of the sample dissolved in 8 mL of phosphate buffer pH 7.0 and 0.2 M were used for analyses.