The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering.
Although it is already known that amine groups increase the cell adhesion and proliferation, the in-
fluence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich
coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF)
capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological
response to the properties of amine layers. As a superior technique for the label-free monitoring of the
cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM
enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related
to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated
surfaces with different properties was studied and evaluated. According to the results, amine-rich
films enhanced the conditions for the cell adhesion and proliferation.
The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering.Although it is already known that amine groups increase the cell adhesion and proliferation, the in-fluence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-richcoatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF)capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biologicalresponse to the properties of amine layers. As a superior technique for the label-free monitoring of thecell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHMenables quantitative phase imaging. From such images, valuable morphological parameters of cells directly relatedto the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreatedsurfaces with different properties was studied and evaluated. According to the results, amine-richfilms enhanced the conditions for the cell adhesion and proliferation.
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