Biochemical estimation
Plasma biochemical parameters: Activity of plasma AST and ALT was estimated following the method of Reitman and Frankel (Reitman and Frankel, 1957) using commercial kit (Span Diagnostics Ltd, Surat, India) and the values were expressed as U/L of plasma. Plasma Total protein was measured using modified Biuret method (Koller, 1984) and values were expressed in gm/dl. Plasma creatinine was measured spectrophotometrically using alkaline picrate method (Bonses and Taussky, 1945).