Then
a few ml of double distilled water was added to the plate to
prevent drying and then incubate at 25 ± 1 oC for a week. At
the end of incubation period, meeting area of Trichoderma –
pathogen hyphae was observed under a light microscope for
the presence of coiling structures for wall disintegration
(Hajieghrari et al., 2008).