The RNA molecular is the basis of violence and pathogenesis of Dengue hemorrhagic fever (DHF) and Dengue Shock Syndrome (DSS) that must to know, to ensure that dengue vaccine development overcome of dengue virus evolution. The mechanisms of vaccine-induced protection need to be clarified in future. The concurrence immunologic target of a neutralizing antibody response to all 4 serotypes in at least 80% of volunteers may not be convenient in all community and clinical settings