The prospects of microalgae biomass as liquid biofuels feedstock in biorefinery concepts are not be restrained by ethanolic fermentation process alone. The fermentation process could be costly and requires high energy thus further contribute to the overall production cost. This concern can be addressed by applying the right fermentation configuration. There are few fermentation configurations employed in current bioethanol production and separate hydrolysis and fermentation (SHF) is the most common method where it involve hydrolysis step before the fermentation. The positive point of this configuration is the hydrolysis step and yeast fermentation operate at their optimum conditions respectively which maximize the fermentable sugar for the yeast to utilize. However, the fermentation process is always upset by high level of glucose content which further reduces the efficiency of yeast ethanolic production. Due to this, additional unit operation is required to further dilute the sugar content for subsequent process. In the other hand, simultaneous saccharification and fermentation (SSF) is able to solve the SHF problems where hydrolysis and fermentation work all together in one reactor. The challenge here is to be able to identify and provide the most conclusive working environment for both the enzyme and yeast. Information on this configuration using lipid-extracted microalgae biomass is scarce thus important in the establishment of microalgae biorefinery industry.