2.4. Analytical techniques
Ethanol production was analyzed by high performance
liquid chromatography (HPLC) on a Varian Pro Star
Autosampler Model 410 liquid chromatograph equipped
with two solvent pumps, a model 210 programmable multiwavelength
detector set at 210nm, a data module, and a
model 320 system controller. The mobile phase was
0.0025N H2SO4. Aliquots of 10 L were injected into an
organic acid column (Varian organic acid column, Cat
#SN 035061) at 22 °C. The Xow rate of the mobile phase
was 0.6mL/min, and the analysis was done under isocratic mode. QuantiWcation of ethanol was done by using standard
ethanol.