Enzyme recycling can effectively increase the rate and yield of the hydrolysis and lower the enzyme cost (Mes-Hartree et al., 1987). Ramos et al. (1993) reported that the enzyme mixture of the commercial Celluclast and Novozym preparation was successfully recycled for five consecutive steps with an elapsed time of 48 h between each recycling step. The efficiency of cellulose hydrolysis decreased gradually with each recycling step