Both sugars and organic acids are found largely sequestered within the vacuole, and form a major contribution to the overall flavor of the fruit and the balance between sugars and organic acid can markedly affect the taste of the fruit. Both sugars and organic acids originate from photosynthetic assimilates. Fruits differ in how they assimilate, accumulates during development and ripening. Some fruits have accumulated the bulk of their carbohydrate prior to the onset of ripening. This is either stored primarily as starch, as in banana, or as sugars, as in tomato. Fruits which accumulate their assimilate prior to ripening can be harvested at the mature green stage and still attain acceptable flavor on ripening. This is important when considering the need for early harvest to optimize shelf life (Tucker, 1993).