Human fingerprints or palm prints have long been used as a leading means for identification and forensic investigation. The most common type of fingerprint evidence, and the one that causes most problems, is the latent fingerprint. Such marks are largely invisible, and generally require some form of physical or chemical treatment to differentiate them from the substrate material. For this purpose fingerprint powders, commonly aluminum flake powders, have been considered particularly effective for over 100 years (Lee and Gaensslen, 2001). Over this period fingerprint powdering has changed little as a detection technique and it is still the most cost-effective method for treating fixed surfaces at a crime scene. During the last 20 years, a wide variety of powders have become commercially available with differing characteristics which render them magnetic, fluorescent or coloured, etc. These dusting agents are based on a variety of materials, both naturally occurring and synthetic, which show affinity for sebaceous gland secretions (sebum) present in finger mark residues. Such residues are usually unsaturated glycerides and cholesterol esters (Champod et al., 2004). Among these new approaches, luminescence detection using a chemical reagent is most prominent since it markedly enhances the visibility of latent fingerprints.