approaches include LMD (Mobile Assay, CO, USA), whom use a custom software application running on a cell phone.51 However, these mobile technologies do not provide the ability to image and process multiple RDT tests simultaneously and require manual handling of both the RDT test and the mobile phone camera. Here, we replace all the external opto-mechanical hardware attachments with customized image proces- sing techniques to create an RDT reader software application capable of detecting and processing multi- ple RDTs imaged using the Google Glass built-in camera under natural illumination conditions along with a hands-free sensing andimage capture interface. Therefore, our RDT reader application on Google Glass retains the mobile interface while allowing for multi- plexed imaging of various types of RDTs from di ff erent manufacturers. Utilizing the existing camera module andthecontinuouswirelessconnectivityoftheGoogle Glass, we combine this hands-free sensing and image capture interface with powerful servers running our custom image processing codes to merge the advan- tages of desktop readers with those of mobile readers. Additionally, our system integrates into a global net- work of lab-on-a-chip applications, allowing for quick retrieval of RDT results through the same Google Glass interface.