The feeding periodicity of three species of fishes in floodplain lagoons of the Macintyre River varied during 24 h cycles, however, peak feeding activity (around midday and late afternoon) was similar among the species studied, despite differences in their trophic position. In contrast, sampling season and site seemed to have a stronger effect on the composition of the diet than time of day. These findings have implications for studies of trophic dynamics and food web structure in floodplain lagoons