CXCL10 mRNA and protein levels in HUVECs
Microarray analysis showed a significant up-regulation of CXCL10 gene expression induced by H9N2 virus and viral particles at 24 h postinfection. We further validated the CXCL10 gene expression at transcriptional level by qRT-PCR and at protein level by ELISA,respectively. The result showed that HUVECs expressed high levels of CXCL10 mRNA and protein after inoculation with H9N2 viruses or viral particles, exhibiting a similar pattern of expression to those analyzed by microarray. As shown in the CXCL10 mRNA expressions by qRT-PCR in HUVECs were significantly increased at 6,12,24 and 36 h postinfection compared with control.