These treatments were distributed in a randomized block containing 3 repeats and each sampling plot
has 10 square meters, the amount of organic manure and chemical fert
ilizer cult ivation converted into
equal quantity nitrogen fertilizer. The deficiency the phosphate (P) and potassium (K) in organic
fertilizers, use Ca(H2PO4)2 and K2SO4 to supply, the planting density is planting distance 20cm and row
space 33cm. The experiment was started on May 1 2009, every 20 days sample and measure stem
diameter, stem length and the dry weight after transplanting
Root activity: Measuring root activity used TTC method.
Photosynthetic rate: Measuring photosynthetic rate used LI-6400 at 10:30 Am to 11:30 Am.
Total glycosides (TG), RA, STV: Measuring stevioside used High Performance Liquid
Chromatography (HPLC).