In this study, two sterile culture media (Table 1), medium type I (Modified Chul3; Inoue et at., 1994) [8] and medium type II (Modified Chul3; Largeau et at., 1980) [9] with the initial pH of 7 were employed. The stock culture with optical density (435 run) of 0.7 was reactivated by transferring 400-mL stock culture into a l-L reactor (I-L Duran bottle) containing 400-mL fresh sterile medium (medium type I or II) and cultivated under the desire temperature (25 DC or 33 ± 3 DC) under
1400 lux light intensity (24-h exposure period). When the OD435 of cell suspension reached 0.7, 400-mL cell suspension was transferred into another 400-mL fresh medium and cultivated under the previously mentioned conditions.