In recent years, the use of microbial biomass and enzymes for decolorization and detoxification of MG-containing wastewater has emerged as a promising solution. Previous studies have demonstrated that many enzymes are involved in MG decolorization and detoxification and those enzymes are often specific to particular species. Biodegradation of MG in yeast has been shown to involve the activities of laccase, lignin peroxidase, aminopyrine N-demethylase, NADH–DCIP reductase and malachite green reductase, of which NADH–DCIP reductase and MG reductase are the most important. Laccase and MG reductase are responsible for MG decolorization and detoxification in Pseudomonas bacteria, the non-basidiomycete filamentous fungi Penicillium pinophilum and Myrothecium roridum. In addition to the enzymes mentioned above, lignin peroxidases, manganese peroxidases (MnP) and laccases in white rot fungi (WRF) are currently the focus of much attention, especially the laccases .