isolated limbal stromal cells along with limbal epithelial cells. In the presence of limbal stromal cells, a
higher absolute number of p63abright cells (p < 0.05) and a higher proportion of K14 positive epithelial
cells were obtained from both ColA and explant tissue cultures. Expansion of the stem/progenitor
population from dispase isolation was more efficient in the form of cell clusters than single cell suspension
based on the absolute number of p63abright cells. Expansion of the stem cell population is similar
in the single cell and cell cluster cultures that are derived from ColA isolation.